1. Makes cells permeable to plasmid DNA

2. Selects for the growth of transformed cells

3. Provides the GFP gene

4. Induces GFP gene expression

5. Causes Green Fluorescent Protein to fluoresce

A. CaCl2

B. Ampicillin

C. pGLO plasmid

D. Arabinose

E. UV Light

1 can be correctly matched with A, Calcium chloride enhances the permeability of plasmid DNA within the cell.  

2 can be correctly matched with B, only the ampicillin-resistant cells or the transformed cell possesses the tendency to grow in the selective media.  

3 can be correctly matched with the pGLO plasmid, the isolation of GFP was done originally from jellyfish, and is usually administered within the pGLO plasmid for examining the expression.  

4 can be correctly matched with D, the expression of GFP is instigated by arabinose as a bidirectional promoter is shared by GFP with the arabinose gene, and thus can get stimulated in its existence.  

5 can be correctly matched with E, in the presence of ultraviolet light, the green fluorescent protein gets fluorescent.