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21.01.2020 •
Biology
A50 î¼l pcr reaction contains two oligonucleotide primers. each primer is present in the pcr reaction at an initial concentration of 1î¼m.
a. how many moles of each primer are present in the pcr reaction?
b. how many molecules of each primer are present in the pcr reaction?
c. if the size of the pcr product in the above reaction is 500 bp, and the pcr reaction yields 2î¼g of the product, how many molecules of each primer are consumed by the pcr reaction?
hint: assume the average molecular weight of a nucleotide base pair is 650 daltons.
d. what ratio (percentage) of each primer was consumed by the pcr reaction
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