Due to the presence of mycolic acids in the cell wall of Mycobacterium spp., the staining procedure can be used as a diagnostic tool to identify this genus.

The question is incomplete as it does not have the option which are:

-acid-fast

-DAPI

-lipo

-Gram

Acid-fast  staining

Explanation:

Acid-fast staining is the technique used to stain and identify the species of the mycobacterium.

During the acid-fast staining, the carbolfuchsin stain is used to color the bacteria as carbolfuchsin solubilizes the lipid of the bacterial wall and the pink stain enters the cell. When these cells are treated with the methylene blue counterstain, the mycobacterium due to the presence of thick cell wall does not allow the entry of the methylene blue as a result of which they appear red o pink.

Thus, acid-fast staining is correct.

The bottom of the trinagle (longest square) is the earliest and the top is the oldest.

Bottom - Top is going

early -old