E. coli strain B is doubly infected with two rII mutants of phage T4. 0.2 ml of a 108 dilution of the progeny is plated on E. coli B and 0.1 ml of a 104 dilution of the progeny is plated on E. coli K. 16 plaques appeared on strain B, 400 on strain K. Calculate the recombination frequency between these two mutations.

Given:

The formula:

→

By putting the values in the above formula, we get

→        

→        

→        

Thus the solution above is the right approach.

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To Find :

The recombination frequency between these two mutations.

Solution :

Formula of calculating recombination frequency is :

Putting all given values in above equation, we get :

Therefore, the recombination frequency between these two mutations is 0.01 or 1 %.

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