In your research on amino acid biosynthesis in e. coli, you have identified three new auxotrophic mutants in a mutagenesis screen. each mutant can grow on minimal medium only if it is supplemented with a specific amino acid, as follows:
lys2 mutants require lysine.
gly3 mutants require glycine.
phe1 mutants require phenylalanine.
as part of your research, you decide to map the locations of the three mutant loci.
to make a rough map of the lys2, gly3, and phe1 loci, you perform an interrupted conjugation experiment between the following two strains:
hfr lys2+ gly3+ phe1+ amps × f- lys2- gly3- phe1- ampr
(note: amps indicates sensitivity to ampicillin. ampr indicates resistance to ampicillin.)
you interrupt conjugation after 1 min, 2 min, and 3 min and plate exconjugants on minimal medium containing ampicillin and various combinations of lysine, glycine, and phenylalanine. you then score the resulting plates for the presence of colonies.
based on the results shown below, what are the possible genotypes of the colonies that grew on each medium? answer the question by dragging one pink label to each pink target, one white label to each white target, and one blue label to each blue target. labels may be used once, more than once, or not at all.
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Ответ:
Option D " all of the above "
Explanation: DNA can be a great source of information when used as a forensic evidence. This technique has many advantages and disadvantages when used as a source of information in case of crime.
Identical twins: There are some common fragments of DNA that is same in identical twins so it can be a difficulty in deciding the criminal.
Not enough of a sample: DNA should be present in a detectable amount to be used an sample for evidence, less than this detectable amount is a waste and cannot be used as a sample for evidence.
Contaminated and degraded sample: If the sample of DNA is contaminated or degraded then the result might be incorrect and might not be used as a sample for forensic evidence.
Hence, the correct answers are all of the above.