Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the below describes the circular piece of DNA from a bacterial cella) restriction enzyme
b) plasmid
c) bacteriophage
d) vector

b) plasmid

Explanation:

A plasmid is an extrachromosomal DNA molecule that found in a cell that replicates independently without the need for chromosomes. A plasmid is small and is also not attached to the chromosomal DNA. Generally, plasmids are a circular piece of double stranded DNA materials that are present in bacteria.

Plasmids are used in a molecular biology laboratory for gene manipulation. they are able to transfer genes to a DNA through DNA recombinant technology and also aid DNA replication in bacteria. Plasmids are also used to study antibiotic resistance.

From the point of view of Charles, he is fearful about this new school all along the story. On the contrary his parents want him to have an upper class atmosphere, to climb the social ladder. It could be said that Charles and his parents might show a certain distance and lack of communication which makes them live the same situation from complete opposite perspectives.