Which of the statements is true about cloning techniques?

The kind of mutation that would affect protein synthesis is one that causes a change in the position of the stop or start codon on the mRNA. This would cause either an incomplete translation or overlengthening of the required proteins on the ribosome. Nonetheless, other mutations such as substitutions would still be translated in the ribosomes to a complete polypeptide. The only implication is that the changes in amino acid content on the polypeptide, due to mutations, will affect how the polypeptide folds into secondary or tertiary proteins which would eventually affect its function.