Answer these three questions to summarize what you have done in this project. 1. How did you model mutations in this project?

When enthalpy change of the reaction is endothermic and entropy change of the reaction is positive, a high T will make reaction more spontaneous (i.e. change in G becomes negative). When T is low, magnitude of enthalpy change will be greater than magnitude of (T x entropy change) and hence change in G will be positive and reaction is non-spontaneous.