Nucleotides polymerize via condensation reactions. the reaction forms a new covalent bond—a phosphodiester linkage, or a phosphodiester bond—between the nucleotides, and a molecule of water is released. where does the phosphodiester bond connect the two nucleotides?

The phosphodiester connects the 3′ carbon of one nucleotide to the 5′ carbon of another nucleotide

Explanation:

The phosphodiester bond is a covalent bond where a phosphate group is attached to adjacent C through an ester bond, which is a consequence of a condensation reaction between the two sugar hydroxyl groups and the phosphate group.

The diester bond between phosphoric acid and two sugar molecules in the DNA and RNA skeleton binds two nucleotides forming polymers known as oligonucleotides.

The phosphodiester bond binds a C3` with a C5` in both DNA and RNA

          (base)1-(sugar)-OH + HO-P(O)2-O-(sugar)-(base)2   >

>   (base)1-(sugar)-O-P(O)2-O-(sugar)-(base)2  

During the reaction of two of the hydroxyl groups in phosphoric acid with a hydroxyl group in two other molecules two ester bonds in a phosphodiester group are formed. A condensation reaction in which a water molecule is lost generates each ester bond.