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BREBRE8932
25.07.2019 •
Mathematics
Difference between expression vector and cloning vector
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Ответ:
Plasmids: Self replicating, circular, extra chromosomal DNA present in bacteria. Plasmids have only one or two copies per cell.
Bacteriophages: Virus infecting bacteria. Bacteriophages have high number per cell, so their copy number is also high in genome.
Cosmids: Hybrid vectors derived from plasmids which contain cos site of lambda phage.
Feature required to facilitate cloning into vector: Origin of replication, Selectable marker, unique restriction sites.
The purpose of cloning vector is often to make numerous copies of the inserted gene.
Expression Vectors: The cloning vector containing suitable expression signals to have maximum gene expression, is called expression vector.
The following expression signals are introduced into gene cloned vectors to get maximum expression:
Insertion of a strong promoter.
Insertion of a strong termination codon.
Adjustment of distance between promoter and cloned gene.
Insertion of transcription termination sequence.
Insertion of a strong translation initiation sequence.
Ответ:
(75 * 3) + 55 + 104 + 85 + 3.
75 * 3, because each race is 75 points.
And now I am confused. "deductions"?
Step-by-step explanation: