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Austrian Empire and France

Explanation:

At that time , these three countries were regarded to be one of the strongest Empire in the world.

The Austrian and French Empire wanted to gain control to the Italian Peninsula since this area connected three continents : Asia, Europe, and Africa. Every single one of them know that gaining control of the Italian Peninsula will massively increased the amount of profit that hey can get from Trades. The wars among them happened on several occasions within the period of 60 years , before eventually Italy won the war in 1559