You conduct a study of romantic love among college students. you ask one student to participate, and she says she is annoyed and offended that you asked her. you have caused her harm, but does this level of harm constitute an ethics violation?

No, because this harm is not greater than that which people encounter in everyday life
People on average got annoyed on even small things such as a missing pen or dirty laundry.
When conducting a study, the refusal to participate from the subject would not be considered as a violation as long as you don't force them any further.

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