Aplasmid vector contains a single ecori site in the polylinker (multiple cloning site). a gene of interest is flanked by two ecori sites, one at each end. you cut the vector and the dna containing your insert with ecori, and ligate the two. how many ecori site(s) is/are present in the recombinant dna molecule and how many band(s) would you see when you run your digest of recombinant dna on a gel? (assume that there is no internal ecori site in the insert dna.)

U should pay attention

The parties have their own sources of funding, some more transparent than others, some more legal than others, focusing only on the legal ones I will list some, the membership fees, militants or affiliates, donations from supporters, loans from banks, and finally and not least, the part that corresponds to them in the PGE of the taxes that we all pay according to the representativeness they have.

In addition to this, the members of the party with some position (councilman, senator, deputy ..) can assign to the party all or part of the wages they receive. It is not unusual for those who combine several positions (councilors who are senators, for example) to make some of their salaries available to the party, although it is also customary for them to have their “expenses” covered with some item from the regional budgets, municipal, or where it comes from.