As a result of its involvement in a reaction, an enzyme as a result of its involvement in a reaction, an enzyme loses energy permanently alters its shape. is unchanged is used up loses a phosphate group

is unchanged

Explanation:

Enzymes are the biological catalysts that serve to speed up the chemical reactions that occur inside the body of living beings. Enzymes are protein in nature and accelerate the rate of the chemical reaction by lowering down the activation energy required for formation of transition state.

Once an enzyme catalyzes one reaction, it is released and catalyzes the next round of chemical reactions. Enzymes are never used up during the reactions.  

For example, once hexokinase enzyme catalyzes phosphorylation of one glucose molecule, it becomes available to catalyze the next round of the same reaction. Hexokinase is not changed or consumed during the reaction .