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1.)., Two populations are said to be isolated if there is no longer any  
GENE FLOW between them.

2.)., Over generations, the GENE POOLS of isolated populations may become more and more different.

3.)., Isolated populations may become genetically different as they adapt to new ENVIRONMENT, or through random processes such as mutation and  GENETIC DRIFT.

4.).,When members of two isolated populations can no longer MATE, successfully, the populations are said to reproductively isolated.

5.).,Reproductive isolation is the final step of SPECIATION, which is the rise of new SPECIES.