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samantha9430
22.04.2021 •
Biology
help?? im done with everything just Explain how losing one part of this food chain will unbalance the whole ecosystem.
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Ответ:
Polymerase Chain Reaction (PCR)
Explanation:
Polymerase chain reaction is a molecular biology procedure/technique used to amplify or multiply specific segments of a DNA. The process makes use of taq polymerase (an enzyme), primers, the DNA of interest and nucleotides.
All the reactants are subjected to repeated heating and cooling cycles during which the DNA of interest is synthesized in multiples. The process can be broken down to 3 basic steps:
Denaturation of the target DNA by heat to separate the DNA strandsBinding of primers to their complementary DNA sequence at lower temperatureSynthesis of new DNA through addition of nucleotides to primers by the taq polymerase. This happens at higher temperature than the second step but lower than the first step.The steps are repeated at the end of each cycle which can last between 2 to 4 hours depending on the length of the target DNA. Newly synthesized DNA at every cycle forms a template for the production of another strand. Hence, multiple copies of the target DNA can be produced within a short period.