How do enzymes affect the activation energy of a reaction

Catalysts lower the Activation energy for reactions. The lower the activation energy for a reaction,the faster the rate. Thus enzymes speed up reactions by lowering activation energy. Many enzymes change shape when substrate ms bind.

Gram-positive microorganisms have a thicker layer of peptidoglycan which traps a greater amount of the gem violet included and upon de-colorizer discharges approximately caught stains. 

Gram-positive cell dividers contain a thick peptidoglycan layer with teichoic acids. Gram-negative cell dividers contain a thin peptidoglycan layer that is encompassed by a thick plasma film. Gram-positive microorganisms will recolor purple in view of their thick peptidoglycan cell divider.