It is important to remove any big air bubbles from the microtubes prior to incubation. Otherwise, the bubbles could:. a. cause contamination of the microtube
b. alter rehydration of the media
c. expand during the incubation and cause the microtube to overflow
d. kill the bacteria

b. alter rehydration of the media

Explanation:

Working in a laboratory implies to know that the air bubbles generally have important negative effects on an experiment, thus being imperative to eliminate them. The air bubbles interfere with several biological experimental procedures including, among others, medium preparation, membrane blotting, chromatography procedures, agar plate, enzyme reactions, running gels, etc. For example, bubbles prevent the cells from attaching to the medium surface, thereby leading to irregular cell growth.