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sawyerfauver
13.08.2021 •
Biology
Suppose that you want to compare the kinetic behavior of the two recently discovered peptidase iso-enzymes PepA and PepB. Your initial studies identified a pentapeptide substrate called Tide5 that appeared to be able to distinguish the kinetic behaviors of PepA and PepB.
At high Tides concentration, the vo (initial velocity) of PepB was greater than PepA, but at low Tide 5 concentration the reverse was true. Which of the following statements is/are consistent with the data stated? More than one answer may apply.
a. The Vmax of PepA must be smaller than that of PepB.
b. The Vmax of both isozymes could be the same, but the Km values must be different.
c. The ratio of Vmas/Km must be smaller for PepA than PepB.
d. The Km of PepA must be lower than that of PepB.
e. The Km of both isozymes could be the same, but the Vmax values must be different.
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