What information would you have to know to determine the toughness of a solid?

how much kinetic energy it absorbed before it broke
its mass and volume
what materials are able to scratch it
its mass

Explanation:

The transcription factor could bind with different affinities to the two closely related  sequences, with the sequence promoting stronger binding leading to higher rates of  transcription initiation.

The binding affinity defines the rate of transcription, stronger the binding affinity, higher the rate of transcription, when lower the binding affinity then lower the transcription rate.

Enhancer regions are binding sequences, or sites, for transcription factors. When a DNA-bending protein binds to an enhancer, the shape of the DNA changes Enhancers: An enhancer is a DNA sequence that promotes transcription.

Finally we can say that enhancer increase the rate of transcription.