Which method would me most efficient to identify where a specific gene is being expressed within the cell?
a. immunofluorescence
b. immunoprecipitation
c. microarray
d. photolithography
e. frap
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Ответ:
b. Immnofluorescence
Explanation:
Immunofluorescence involves the reaction of a fluorescently labelled antibody to an expressed gene or protein making it specific. A microscope is used to locate the expressed gene via the fluorescent dye activated by light of a certain wavelength. It is usually done to analyze intact cells or tissue therefore it is easier to localize the gene expression.
Ответ:
Compound microscope is commonly used in the schools and colleges. It has two lenses, the objective lens and the ocular lens. It provides a magnification of 1500X. Eyepiece lens is of 10X or 15X power. It is used to observe bacterial, protozoa, various cells, etc.
Dissecting Microscope It is also called stereo microscope. Its magnification power is less than the other microscope. It gives a 3 dimensional picture. Due to the low magnifying power they are used to observe little bigger objects than other microscopes. They are used in the surgeries, dissection, forensic science etc.