You design primers for colony PCR with one primer binding to your gRNA insert (20 bp), and 1 primer binding to a region of your plasmid ~430 base pairs away. Your plasmid vector is pML104, which is 11,240 base pairs. In colony PCR, a positive result (indicating a colony likely carries a pML104 plasmid with the gRNA inserted) would be a PCR product that is:
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Ответ:
Thymine: 27%. Guanine: 23%
Explanation:
Since Adenine pairs with Thymine they have the same percentage of composition.
Also, since Cytosine pairs with Guanine they have the same percentage