You want to engineer a eukaryotic gene into bacterial colony and have it expressed. what must be included in addition to the coding exons of the gene

A bacterial promoter sequence must be included. The coding exons control the actual production of the protein that the gene codes for; meanwhile, the promoter sequence serves to initiate transcription of the specific genes. Promoters are included upstream of the DNA molecule at the start site of the gene.

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respiratory failure is a condition in which your blood doesn't have enough oxygen or has too much carbon dioxide. your organs, such as your heart and brain, need this oxygen-rich blood to work well. another part of breathing is removing the carbon dioxide from the blood and breathing it out

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