A new student is planning to use thin layer chromatography (TLC) for his research project. After setting up the apparatus the student forgets to place a lid on the TLC jar. He obtains poor results after running the TLC experiment. Which of the following can best explain his bad results?
A. The open system evaporates the solvent on the TLC plate
B. The open system prevents the evaporation of the solvent in the solution
C. The open system prevents the evaporation of the solvent on the TLC plate
D. The open system evaporates the solvent in the solution
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Ответ:
The open system evaporates the solvent in the solution
Explanation:
An open system is a system in which exchange of materials and energy can occur. If a TLC set up is left open, then the set up constitutes an open system.
During TLC, the sample is dotted on the plate and inserted into a suitable solvent. The solvent moves up the plate and achieves the required separation of the mixture.
Most of these solvents used used TLC are volatile organic compounds. Therefore, if the TLC set up is left open, the solvent will evaporate leading to poor results after running the TLC experiment.
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