During the rGFP purification experiment, the instructor will have to make breaking buffer for the students to use. This buffer contains 150mM NaCl. Given a bottle of crystalline NaCl (M.W. = 40g/mole), describe how you would make 500ml of 150mM NaCl. Include the type and sizes of any measuring vessels that you use to make the solution.
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Ответ:
dealing with a buffer
use the formula C = n/V...........1
therefore say n = m/Mr.........2
then substitute equation 2 into equation 1
∴ CV = m/Mr
then m = CVMr
Since we are looking for mass of Nacl to make a buffer solution.
m = 150×
m
× 500×
l × 40 g/mol
mass of Nacl = 3g
Explanation: You first weight out 3g of 150mM of NaCl and then dissolve it into a 1000ml beaker dilute it using a volume of 500ml of water to make volume.
Ответ:
Utah I believe, if not then its cali
Explanation: