kprincess16r
12.02.2020 •
Chemistry
The molar extinction coefficient of cytochrome P450, one of the compounds involved in photosynthesis, at 522 nm is 291 L mol-1cm-1. When light of that wavelength passes through a cell of length 6.5 mm containing a solution of P450, 39.8 % of the light was absorbed. What is the molar concentration of the solution?
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Ответ:
0.001165 mol/L is the molar concentration of the solution.
Explanation:
Using Beer-Lambert's law :
Formula used :
where,
A = absorbance of solution
C = concentration of solution
l = path length
T = Transmittance
= incident light
= transmitted light
= molar absorptivity coefficient
We have :
Transmittance of the light = 100% - 39.8 % = 60.2 % = 0.602
Absorbance of the light :
Molar absorptivity coefficient =
Length of the cell = l 6.5 mm = 0.65 cm ( 1 mm = 0.1 cm)
Now put all the given values in the above formula, we get the concentration of solution :
0.001165 mol/L is the molar concentration of the solution.
Ответ: