![heysorryguys](/avatars/46513.jpg)
heysorryguys
08.03.2021 •
Biology
You will calculate the concentrations of the components required for a chromium release
cytotoxicity assay. Target cells are labelled with radioactive chromium and incubated with CD8
effector cells for 6 hours. The release of the radioactivity can be measured as counts per minute
(cpm) and the amount of target cell killing calculated.
Your assay has radioactively labelled tumour cell targets and you will add your killer cells, called
effectors, to the targets.
The effector cells will be added to the target cells in increasing numbers to give effector:target
ratios of 40:1, 20:1, 10:1 and 5:1.
Part 1
a) You have radioactively labelled target cells at a concentration of 2 x 10 per ml.
You need 1 x
10 cells in each well, which will be constant, for your assay.
Volume of target cells required()ul
Solved
Show answers
More tips
- F Food and Cooking How to Cook Spaghetti Right – Secrets and Tips...
- P Philosophy Personal attitude towards Confession: how to prepare and undergo the procedure correctly?...
- H Health and Medicine Flu: How to Recognize It by the First Symptoms?...
- F Food and Cooking How to Sober Up Quickly? Important Facts and Tips...
- H Health and Medicine How to Properly Take a Blood Sugar Test?...
- H Health and Medicine Simple and Effective: How to Get Rid of Cracked Heels...
- O Other How to Choose the Best Answer to Your Question on The Grand Question ?...
- L Leisure and Entertainment History of International Women s Day: When Did the Celebration of March 8th Begin?...
- S Style and Beauty Intimate Haircut: The Reasons, Popularity, and Risks...
- A Art and Culture When Will Eurovision 2011 Take Place?...
Answers on questions: Biology
- S Social Studies The three-fifths compromise a. increased the power of the free states by counting only 3/5 of the slaves instead of none of them. b. increased the power of slave states by...
- H History An economic arrangement in which serfs lived and worked on a lord’s estate in return for food and protection could best describe the...
Ответ: