One g of fecal matter was suspended in 99 ml saline solution (tube 1). After mixing gently but thoroughly, one ml from tube 1 was removed and added to tube with 99 ml saline solution (tube 2). One ml suspension from tube 2 was played in MacConkey agar. After 24 h incubation, 30 red colonies and 50 colorless colonies were counted. Determine cfu/g Gram-negative bacteria in the fecal sample. Your answer must be in scientific notation such as 1.0 x 10^2. Round your answer to the nearest 10th. For example, your answer is 1.675 x 10^5 cfu/g, type 1.7 x 10^5 cfu/g. Put a space before and after the x. The x is lower case.

the cfu/g Gram-negative bacteria in the fecal sample is C = 3.0 × 10^3

Explanation:

We know that; Gram negative bacteria looks  pale reddish in color under a light microscope from Gram staining.

therefore

There are 30 red bacterial colonies counted.

1 mL of from tube 1 was removed and added to tube with 99 mL saline (tube 2) dilution is 1/100.

transferred volume  into the plate is 1 mL.

Now, we have to determine the cfu/g Gram-negative bacteria in the fecal sample

Formula to calculate CFU/g bacteria in fecal sample is expressed as;

C = n/(s×d )

where C is concentration (CFU/g) , n is number of colonies , s is volume transferred to plate , d is dilution factor.

so we substitute

C = 30 / ((1/100) × 1)

C = 30 / 0.01

C = 3000

C = 3.0 × 10^3

THERFERE, the cfu/g Gram-negative bacteria in the fecal sample is C = 3.0 × 10^3

So what does the cell theory actually mean? Let’s break it down. The first part of the cell theory states that all living things are made up of cells. Anything that’s alive, from bacteria to plants to humans, is composed of cells. And what are cells? The literal definition is a cell is a group of organelles surrounded by a thin membrane.

The cell theory definition states that cells are the building blocks of life. Cells both make up all living things and run the processes needed for life. Your hair, skin, organs, etc. are all made up of cells. In fact, each person is estimated to be made up of nearly 40 trillion cells! Each part of a cell has a different function, and your cells are responsible for taking in nutrients, turning nutrients into energy, removing waste, and more. Basically, everything your body does, it does because cells are directing the action!

The third part of the cell theory definition states that all cells come from preexisting cells. This means that cells don’t just appear out of thin air (known as “spontaneous generation”). New cells are always made from current cells. This means that all current life on the planet is descended from the very first cells, which first made an appearance on Earth roughly 3.5 billion years ago. Cells have been replicating themselves continuously ever since.

And what does the newer part of the cell theory state? Part four refers to the fact that, in all living cells, energy is continuously transformed from one type to another. Examples of these processes include photosynthesis (where plant cells convert light energy into chemical energy) and cellular respiration (where both plant and animal cells convert glucose into energy). Part five refers to DNA and the fact it is passed from parent cell to child cell. Finally, part six of the cell theory tells us that all cells are made up of the same chemicals: water, inorganic ions, and organic molecules.

As our scientific knowledge has increased over time, additional parts have been added to the theory. Schleiden and Schwann, as well as Virchow, are generally seen as the founders of the cell theory, due to their pioneering scientific work in the 1800s. The cell theory is important because it affects nearly every aspect of biology, from our understanding of life and death, to how we manage diseases, and more.

Explanation:

um i copied some random article online so tbh i dont suggest u use this