emmilicious
19.06.2020 •
Biology
Students have expressed a protein according to the BASIL protocols. They collected a pre-induction sample, a post-induction sample, and a sample following an affinity column purification. The samples were analyzed by SDS-PAGE followed by Coommassie Blue staining. Which lane corresponds to which sample?
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Ответ:
The first lane with the protein band is the post-induction sample, i.e., the lane with the sample of interest.
Explanation:
Antibody binding is specific and will only bind to whichever protein it has been produced for particularly, which in this case would be the post induction sample.
Ответ:
hope this helps